| Name | Team | Time | FINA | Imp | |||
|---|---|---|---|---|---|---|---|
| 1 | Hunter Mehring |
 Principia
|
51.71 | 619 | – | PB | |
| 2 | Houfu Yan |
 Grinnell
|
52.93 | 577 | -0.2% | ||
| 3 | Luke Gartner |
 NWU
|
54.33 | 534 | -1.3% | ||
| 4 | Robert Bonanno |
 Carroll
|
55.98 | 488 | +0.4% | SB | |
| 5 | Sam Trofholz |
NWU
|
56.25 | 481 | -0.7% | ||
| 6 | Brody Beyer |
Carroll
|
56.55 | 473 | -0.3% | ||
| 7 | Ben Grubb |
Grinnell
|
57.07 | 460 | -0.1% | ||
| 8 | Tyler Marine |
 Simpson
|
57.18 | 458 | -0.2% | ||
| 9 | Jackson Thompson |
Grinnell
|
57.38 | 453 | +0.3% | SB | |
| 10 | Chris Hernandez |
 Monmouth
|
57.87 | 442 | +3.4% | PB | |
| 11 | Jake Hall |
Monmouth
|
57.92 | 440 | -0.7% | ||
| 12 | Jack P. Lutter |
 Ripon
|
58.15 | 435 | – | ||
| 13 | Max Stahl |
 Lawrence
|
58.46 | 428 | +0.4% | SB | |
| 14 | Albert Ford |
Grinnell
|
58.48 | 428 | +2.1% | PB | |
| 15 | James Park |
Grinnell
|
58.96 | 418 | +4.0% | SB | |
| 16 | Braly Keller |
NWU
|
59.50 | 406 | – | ||
| 17 | John Wilcox |
Carroll
|
59.57 | 405 | -1.9% | ||
| 18 | Johan Vizoso-Marino |
NWU
|
1:00.07 | 395 | -0.4% | ||
| 19 | Benjamin Heller |
Grinnell
|
1:00.24 | 392 | – | ||
| 20 | Stephen Duhaney |
Grinnell
|
1:00.69 | 383 | – | ||
| 21 | Mike Weisenfelder |
 Illinois College
|
1:01.09 | 375 | – | ||
| 22 | Zachary Souza |
Grinnell
|
1:01.24 | 373 | – | ||
| 23 | Michael Beckman |
Carroll
|
1:01.88 | 361 | +2.7% | SB | |
| 24 | Aalton Lande |
Grinnell
|
1:02.82 | 345 | -1.6% | ||
| 25 | Brendan Atwood |
Monmouth
|
1:02.98 | 343 | +1.9% | PB | |
| 25 | Martin Ambre |
Illinois College
|
1:02.98 | 343 | – | PB | |
| 27 | Tutis Rudzinskas |
Ripon
|
1:03.21 | 339 | +0.4% | SB | |
| 28 | Andrew Boyd |
Principia
|
1:03.40 | 336 | +0.1% | SB | |
| 29 | Ben Allen |
Illinois College
|
1:03.54 | 334 | -4.9% | ||
| 30 | Tristan Knoth |
Grinnell
|
1:03.74 | 331 | – | ||
| 31 | Martin Toney |
Grinnell
|
1:04.18 | 324 | +0.6% | PB | |
| 32 | Kahlil Epps |
Grinnell
|
1:04.79 | 315 | -0.3% | ||
| 33 | Brady Burden |
Illinois College
|
1:05.09 | 310 | – | ||
| 34 | Alex Johnson |
Grinnell
|
1:07.23 | 282 | – | ||
| 35 | Jakob Struble |
Lawrence
|
1:07.76 | 275 | – | ||
| 36 | Christopher Morton |
Illinois College
|
1:11.05 | 239 | – | ||
| 37 | Ethan Moscoso |
Grinnell
|
1:11.95 | 230 | -1.1% | ||
| 38 | Jonathan Gilmour |
Grinnell
|
1:14.74 | 205 | – | ||
| 39 | William Rebelsky |
Grinnell
|
1:20.83 | 162 | – | ||
| 40 | Dhruv Bakshi |
Grinnell
|
1:23.07 | 149 | – | ||
