| Name | Time | Date | Meet |
|---|---|---|---|
| Natalie Cox | 30.56 | Nov. 18, 2023 | Wabash Pebworth Invitational |
| Natalie Cox | 30.90 | Feb. 10, 2023 | Southern Athletic Association Championships |
| Natalie Cox | 31.03 | Nov. 19, 2022 | Wabash Pebworth Invitational |
| Jacquelyn Engel | 31.53 | Feb. 15, 2019 | Southern Athletic Association Championships |
| Meredith Angel | 32.55 | March 9, 2007 | 2007 NCAA Division III Womens Championships |
