| Name | Time | Date | Meet |
|---|---|---|---|
| Dani Lacy | 52.47 | Dec. 7, 2013 | Transylvania Invitational |
| Jane Tsivitse | 53.04 | Feb. 16, 2013 | 2013 North Coast Athletic Conference Championships |
| Dani Lacy | 53.08 | Nov. 30, 2012 | DePauw Invitational |
| Jane Tsivitse | 53.37 | Feb. 18, 2012 | North Coast Athletic Conference Championships |
| Jane Tsivitse | 53.98 | Dec. 2, 2011 | DePauw Invitational |
| Sinjin McNicholl | 54.07 | Feb. 13, 2010 | North Coast Athletic Conference Championships |
| Cecilia Wille | 54.53 | Nov. 21, 2008 | Wittenberg @Ohio Northern |
| Kristin Elber | 55.61 | Nov. 1, 2008 | Wittenberg @Wooster |
