| Time | Date | Name | Meet |
|---|---|---|---|
| 43.80 | March 12, 2021 | JULIAN ITURBE | Michigan Intercollegiate Athletic Association Men's Championships |
| 44.12 | Jan. 29, 2021 | JULIAN ITURBE | Calvin vs. GVSU |
| 44.30 | Dec. 7, 2019 | JULIAN ITURBE | Calvin Invitational |
| 44.88 | Nov. 30, 2017 | Jared Britton | Calvin Invitational |
| 45.43 | Feb. 18, 2017 | Jared Britton | Michigan Intercollegiate Athletic Association Championships |
| 45.76 | March 19, 2016 | Brandon Muma | NCAA Division III Championships |
| 45.77 | Feb. 15, 2014 | Andrew Mitchell | Michigan Intercollegiate Athletic Association Championships |
| 46.06 | Dec. 7, 2013 | Andrew Mitchell | Calvin College Invitational |
| 46.23 | March 22, 2008 | John Boumgarden | NCAA Division III Mens Championship |
