| Time | Date | Name | Meet |
|---|---|---|---|
| 50.77 | Feb. 17, 2023 | Liam Noble | Minnesota Intercollegiate Athletic Conference Championships |
| 50.88 | Feb. 18, 2022 | Joe Koller | Minnesota Intercollegiate Athletic Conference Championships |
| 51.21 | Feb. 17, 2022 | Joe Koller | Minnesota Intercollegiate Athletic Conference Championships |
| 51.25 | Feb. 19, 2016 | Braden McCormack | Minnesota Intercollegiate Athletic Conference Championships |
| 51.28 | Feb. 13, 2014 | Braden McCormack | Minnesota Intercollegiate Athletic Conference Championships |
| 52.09 | Feb. 15, 2013 | Braden McCormack | Minnesota Intercollegiate Athletic Conference Championships |
| 52.73 | Feb. 19, 2010 | Andrew Happe | Minnesota Intercollegiate Athletic Conference Championships |
| 53.37 | March 17, 2006 | Casey Larson | NCAA Division III Mens Championships |
