| Time | Date | Name | Meet |
|---|---|---|---|
| 42.77 | Feb. 27, 2016 | Andrew Marsh | Big XII Conference Championships |
| 42.92 | March 1, 2014 | Timothy Squires | Big XII Conference Championships |
| 43.93 | March 2, 2013 | Ross Glegg | Big 12 Conference Championships |
| 43.95 | Feb. 18, 2012 | Timothy Squires | Big East Conference Championships |
| 44.20 | Feb. 20, 2010 | Brad Eichenseer | Big East Championships |
| 44.39 | March 15, 2007 | Zoltan Povazsai | NCAA Division I Mens Championships |
