| Time | Date | Name | Meet |
|---|---|---|---|
| 56.19 | Feb. 17, 2024 | Harrison Cerone | W&L Last Chance Meet |
| 56.56 | Feb. 9, 2024 | Harrison Cerone | Old Dominion Athletic Conference Championships |
| 56.68 | Feb. 10, 2023 | Todd Echols | Old Dominion Athletic Conference Championships |
| 56.76 | Feb. 12, 2022 | Steven Warren | Old Dominion Athletic Conference Championships |
| 56.97 | Feb. 8, 2020 | Steven Warren | Old Dominion Athletic Conference Championships |
| 57.73 | Feb. 16, 2019 | Steven Warren | Old Dominion Athletic Conference Championships |
| 57.96 | Feb. 11, 2017 | Ethan Fischer | Old Dominion Athletic Conference Championships |
| 58.09 | Feb. 17, 2012 | Ian Dexter | Bluegrass Mountain Conference Championships |
| 58.60 | Feb. 19, 2010 | Ian Dexter | Bluegrass Mountain Valley Championships |
| 58.99 | Dec. 3, 2009 | Ian Dexter | Virginia Invitational |
| 1:03.18 | Nov. 6, 2009 | Matthew Childers | Wingate vs. Catawba, Rollins, Washington & Lee |
| 1:03.73 | Oct. 16, 2009 | Robert Grattan | Washington & Lee vs Mary Washington |
