| Name | Time | Date | Meet |
|---|---|---|---|
| Chloe Dewberry | 29.10 | Feb. 12, 2022 | Old Dominion Athletic Conference Championships |
| Chloe Dewberry | 29.86 | Feb. 8, 2020 | Old Dominion Athletic Conference Championships |
| Chloe Dewberry | 30.83 | Nov. 23, 2019 | Randolph-Macon/Yellow Jacket Invitational |
| Chloe Dewberry | 31.06 | Nov. 16, 2019 | Virginia Wesleyan vs. Salisbury |
| Kiersten Lee | 31.50 | Oct. 12, 2019 | ODAC Relays |
| Francesca Lanuza | 35.19 | Oct. 21, 2017 | Virginia Wesleyan vs. Randolph |
| Hayley Heath | 36.82 | Oct. 14, 2017 | ODAC Relays |
