| Name | Time | Date | Meet |
|---|---|---|---|
| Kacey McKenna | 50.23 | March 22, 2024 | NCAA Division I Womens Championship |
| Kacey McKenna | 50.49 | Feb. 23, 2024 | B1G Women's Championships |
| Brooklynn Snodgrass | 51.05 | March 21, 2014 | 2014 NCAA Division I Womens Championships |
| Kateryna Zubkova | 51.08 | Oct. 31, 2008 | Texas, Indiana @Michigan |
| Kateryna Zubkova | 52.02 | March 21, 2008 | 2008 NCAA Division I Womens Championships |
| Leila Vaziri | 52.88 | March 9, 2007 | 2007 NCAA Division I Womens Championships |
| Leila Vaziri | 53.14 | March 8, 2007 | 2007 NCAA Division I Womens Championships |
