| Name | Time | Date | Meet |
|---|---|---|---|
| Christine Jensen | 23.81 | Feb. 22, 2019 | Big Ten Conference Championships (W) |
| Christine Jensen | 24.21 | Nov. 16, 2018 | Indiana Invitational |
| Gia Dalesandro | 24.37 | Dec. 4, 2015 | Hoosierland Invitational |
| Kateryna Fesenko | 24.68 | Dec. 4, 2009 | AT&T Short Course National Championships |
| Marie Smailis | 25.23 | Dec. 5, 2008 | ConocoPhillips Short Course Championships |
| Annica Lofstedt | 25.73 | March 9, 2007 | 2007 NCAA Division I Womens Championships |
