| Name | Time | Date | Meet |
|---|---|---|---|
| Vitoria Issaho dos Santos | 28.52 | Feb. 14, 2018 | Great Midwest-Mountain East Conference Championship |
| Vitoria Issaho dos Santos | 28.76 | Feb. 8, 2017 | 2017 Bluegrass Mountain Championships |
| Kenya Paiz | 29.33 | Nov. 19, 2016 | Corbiere-Merion Invitational |
| Autumn Wunderlich | 29.50 | Oct. 19, 2013 | Davis and Elkins vs. Alderson-Broaddus |
| Autumn Wunderlich | 30.10 | Oct. 12, 2013 | West Virginia Invitational |
| Melissa Pusanik | 39.51 | Oct. 27, 2012 | Georgia Southern vs. Davis and Elkins, SCAD |
