| Time | Date | Name | Meet |
|---|---|---|---|
| 23.62 | Dec. 6, 2019 | Denise Quentin | Zippy Invitational |
| 23.88 | Feb. 23, 2019 | Denise Quentin | Kenyon Fast Chance Invitational |
| 23.98 | Feb. 13, 2019 | Denise Quentin | Great Midwest-Mountain East Conference Championship |
| 24.29 | Dec. 5, 2013 | Stephanie Brookens | Wooster Invitational |
| 24.43 | Feb. 7, 2013 | Stephanie Brookens | Great Lakes Intercollegiate Athletic Conference |
| 25.12 | Oct. 26, 2012 | Stephanie Brookens | Defiance vs. Malone, Mount Union |
| 25.77 | Oct. 13, 2012 | Stephanie Brookens | Denison vs. Malone |
| 26.59 | Oct. 29, 2011 | Allison Byrd | West Virginia Wesleyan vs. Malone, Oberlin |
| 27.21 | March 4, 2011 | Chelsea Perry | NAIA National Championships |
| 27.43 | Nov. 19, 2010 | Chelsea Perry | John Carroll Invite |
| 27.93 | Oct. 15, 2010 | Michelle Wagner | Christian College Invitational |
| 28.98 | Feb. 20, 2010 | Ashley Kuhn | Last Chance Meet: Kenyon |
