| Name | Time | Date | Meet |
|---|---|---|---|
| Emma Paulson | 50.35 | March 19, 2016 | 2016 NCAA Division III Championships |
| Rebecca Ney | 51.01 | Feb. 20, 2010 | Minnesota Intercollegiate Athletic Conference Championships |
| Rebecca Ney | 53.72 | Dec. 5, 2009 | UW-River Falls Falcon Invite - Red Division |
| Rebecca Ney | 54.38 | Nov. 21, 2009 | Ahlman Invitational |
| Rebecca Ney | 55.05 | Dec. 6, 2008 | 2008 Falcon Invite |
