| Name | Time | Date | Meet |
|---|---|---|---|
| Sydney Zupan | 54.17 | Feb. 17, 2017 | 2017 Missouri Valley Conference Championships |
| Anna Ahlin | 54.38 | Feb. 14, 2014 | 2014 Missouri Valley Conference Championships |
| Anna Ahlin | 54.51 | Feb. 13, 2014 | 2014 Missouri Valley Conference Championships |
| Jordan Hawkins | 54.58 | Feb. 13, 2009 | 2009 Missouri Valley Conference Championships - Day Two - Finals |
| Chelsea Dirks-Ham | 58.11 | Nov. 15, 2008 | 2008 Henderson State Invitational |
| Chelsea Dirks-Ham | 58.42 | Nov. 7, 2008 | Lindenwood@Missouri State |
