| Time | Date | Name | Meet |
|---|---|---|---|
| 51.26 | Feb. 22, 2024 | Kamryn Cannings | ASUN Championships |
| 51.72 | March 21, 2014 | Jessica Reinhardt | NCAA Division I Womens Championships |
| 52.14 | Feb. 21, 2014 | Jessica Reinhardt | Coastal Collegiate Swimming Association Championships |
| 52.87 | March 3, 2013 | Jessica Reinhardt | Ohio State Last Chance Meet |
| 53.18 | Feb. 22, 2013 | Jessica Reinhardt | Coastal Collegiate Swimming Association Championships |
| 54.77 | Feb. 17, 2012 | Bethany Wakeley | Coastal Collegiate Swimming Association Championships |
| 57.31 | Feb. 18, 2011 | Bethany Wakeley | Coastal Collegiate Swimming Association Championships |
| 59.49 | Oct. 8, 2010 | Bryeanna Ravettine | Radford vs. Liberty, North Carolina A&T |
