| Time | Date | Name | Meet |
|---|---|---|---|
| 48.00 | March 8, 2024 | Jokūbas Jankauskas | NAIA National Championships |
| 48.15 | March 3, 2023 | Jokūbas Jankauskas | NAIA National Championships |
| 49.24 | Dec. 1, 2022 | Jokūbas Jankauskas | Wooster Invitational |
| 49.83 | March 1, 2013 | Zachary Johnson | NAIA Championships |
| 50.90 | March 2, 2012 | Robert Miller | NAIA Championships |
| 51.11 | March 4, 2011 | Robert Miller | NAIA National Championships |
| 52.37 | Nov. 18, 2010 | Robert Miller | Western Kentucky Invitational |
| 52.63 | March 5, 2010 | Andrzej Lenard | NAIA National Championships |
| 53.16 | Nov. 20, 2009 | Robert Miller | Hilltopper Invitational |
| 55.87 | Oct. 17, 2009 | Andrzej Lenard | Kentucky vs. Arkansas, Cumberland, Southern Illinois |
