| Time | Date | Name | Meet |
|---|---|---|---|
| 52.22 | Nov. 16, 2023 | Johan Cue Carrillo | Mizzou Invitational |
| 52.28 | Feb. 24, 2023 | Johan Cue Carrillo | Summit League Championships |
| 52.77 | March 19, 2021 | Gerald Brown | NCAA Division II Championships |
| 52.87 | Feb. 22, 2019 | Justin Winnett | New South Conference Championships |
| 53.25 | Dec. 17, 2018 | Justin Winnett | Delta State Christmas Invitational |
| 53.46 | Dec. 18, 2017 | Justin Winnett | Delta State Christmas Invitational |
| 53.71 | March 11, 2016 | Mateusz Pacholczyk | NCAA Division II Championships |
| 53.96 | March 13, 2015 | Mateusz Pacholczyk | NCAA Division II Championships |
| 54.18 | Dec. 14, 2014 | Mateusz Pacholczyk | Delta State Invitational |
| 54.22 | Feb. 21, 2014 | Mateusz Pacholczyk | New South Conference Championships |
| 55.08 | Dec. 15, 2013 | Mateusz Pacholczyk | Delta State Christmas Invitational |
| 56.15 | Nov. 30, 2012 | Reinis Rozensteins | Missouri Invitational |
| 56.57 | March 5, 2010 | Sergio Molina | NAIA National Championships |
| 56.98 | Feb. 19, 2010 | Sergio Molina | Liberal Arts Championships |
| 57.02 | Jan. 23, 2010 | Sergio Molina | Illinois Tech/St. Louis U/Missouri State/Lindenwood U Quad |
| 58.27 | Nov. 21, 2009 | Sergio Molina | Washington University Invitational |
| 58.39 | Oct. 10, 2009 | Sergio Molina | Show-Me Showdown |
| 1:01.85 | Jan. 16, 2009 | Aaron Saule | Washington University Invitational |
