| Name | Time | Date | Meet |
|---|---|---|---|
| Connor Lee | 45.52 | Feb. 24, 2023 | Ivy League Championships (M) |
| Connor Lee | 46.12 | Feb. 28, 2020 | Ivy League Championships (M) |
| Mike Dominski | 47.34 | March 2, 2012 | Ivy League Mens Championships |
| Chris Pool | 47.41 | March 6, 2009 | 2009 Eastern Intercollegiate Swim League Championships - Day Two - Prelims |
| Chris Pool | 47.92 | Dec. 5, 2008 | ConocoPhillips Short Course Championships |
| Chris Pool | 48.35 | March 7, 2008 | 2008 EISL Championship |
| Chris Pool | 48.78 | Feb. 23, 2007 | 2007 EISL Championships |
| Alex Nash | 49.38 | Feb. 28, 2002 | 2002 Men's EISL Championships |
