| Time | Date | Name | Meet |
|---|---|---|---|
| 52.72 | March 2, 2019 | Jacklyn Smailis | Boiler-Make-It Qualification |
| 52.81 | Feb. 18, 2011 | Allison Smith | Big Ten Conference Womens Championships |
| 53.20 | March 19, 2010 | Allison Smith | NCAA Division I Womens Championships |
| 53.62 | Feb. 19, 2010 | Allison Smith | Big Ten Women's Championships |
| 55.14 | Oct. 23, 2009 | Allison Smith | Miami-OH vs. Purdue |
| 57.21 | Nov. 23, 2008 | Katie Beck | Texas A&M Invitational |
