| Time | Date | Name | Meet |
|---|---|---|---|
| 49.28 | March 11, 2023 | Hunter Gubeno | CSCAA National Invitational Championships |
| 49.74 | March 12, 2022 | Dominic Polling | CSCAA National Invitational Championship |
| 51.23 | Nov. 19, 2016 | Eitan Holder | Bearcat Midseason Invitational |
| 52.89 | Feb. 20, 2016 | Connor Brady | American Conference Championships |
| 53.64 | Oct. 16, 2015 | Tyler Roehr | Cincinnati vs. Cleveland State, Xavier |
| 54.87 | Sept. 11, 2015 | Ryan DePietro | Cincinnati Red & Black Intrasquad |
