| Time | Date | Name | Meet |
|---|---|---|---|
| 46.32 | Feb. 25, 2021 | Blake Hanna | American Athletic Conference Championships |
| 47.50 | Feb. 21, 2020 | Blake Hanna | American Athletic Conference Championships |
| 48.03 | March 1, 2019 | Joseph Puglessi | American Athletic Conference Championships |
| 48.53 | Feb. 20, 2009 | Jeremy Peter | Big East Championships - Day Three - Finals |
| 50.84 | Nov. 15, 2008 | Jeremy Peter | Pittsburgh @Cincinnati |
| 51.54 | Nov. 7, 2008 | Jeremy Peter | Cincinnati @Ohio State |
