| Name | Time | Date | Meet |
|---|---|---|---|
| Gabriela Herbreder | 25.08 | Feb. 17, 2023 | Atlantic 10 Conference Championships |
| Toni Rafferty | 25.88 | Feb. 21, 2020 | Atlantic 10 Conference Championships |
| Emily Wolbert | 26.13 | March 14, 2019 | CSCAA National Invitational Championship |
| Emily Wolbert | 26.44 | Feb. 22, 2019 | Atlantic 10 Conference Championships |
| Emily Wolbert | 27.11 | Nov. 17, 2018 | Bucknell Invitational |
| Lianne McCluskey | 27.53 | Feb. 2, 2013 | La Salle vs. Pennsylvania |
