| Name | Time | Date | Meet |
|---|---|---|---|
| Will Modglin | 44.20 | March 29, 2024 | NCAA Division I Mens Championships |
| John Shebat | 44.35 | March 24, 2017 | 2017 NCAA Division I Mens Championships |
| John Shebat | 44.58 | March 23, 2017 | 2017 NCAA Division I Mens Championships |
| Kip Darmody | 45.03 | March 28, 2014 | 2014 NCAA Division I Mens Championships |
| Taylor Taylor | 45.17 | Oct. 31, 2008 | Texas, Indiana @Michigan |
| Tom Sacco | 46.19 | March 15, 2007 | 2007 NCAA Division I Mens Championships |
| Colin Gaffney | 50.10 | March 6, 2003 | American Short Course Championships |
