| Time | Date | Name | Meet |
|---|---|---|---|
| 43.20 | March 16, 2024 | Nico Jacinto | NCAA Division II Championship |
| 43.66 | Feb. 12, 2022 | Ruben Leeuwen | Great Lakes Valley Conference Championships |
| 44.10 | Feb. 15, 2020 | Guillaume Bolivard | Great Lakes Valley Conference Championships |
| 44.28 | Nov. 23, 2019 | Guillaume Bolivard | Phoenix Fall Classic |
| 44.48 | March 11, 2017 | Adam Wise | NCAA Division II Championship Festival |
| 44.55 | Feb. 11, 2017 | Adam Wise | Great Lakes Valley Conference Championships |
| 44.72 | Feb. 14, 2015 | Victor Tarin | Great Lakes Valley Conference Championships |
| 44.97 | Feb. 15, 2014 | Victor Tarin | Great Lakes Valley Conference Championships |
| 45.33 | Nov. 24, 2013 | Victor Tarin | House of Champions Invitational |
| 45.85 | Oct. 19, 2013 | Victor Tarin | Lewis vs. Hillsdale |
| 46.72 | Oct. 18, 2013 | Aidan Brennan | Lewis vs. Missouri S & T |
| 47.16 | Jan. 12, 2013 | Bryan Wiener | Carthage vs. Lewis |
| 47.50 | Dec. 2, 2011 | Derick Carlson | Carthage CollegeSwimming Classic |
| 47.78 | Feb. 12, 2011 | Allan Sliva | Great Lakes Intercollegiate Athletic Conference Championships |
| 47.91 | Nov. 21, 2009 | Allan Sliva | Phoenix Fall Invitational (University of Chicago) |
| 48.90 | Nov. 11, 2007 | Allan Sliva | USA Swimming Toyota Grand Prix |
