| Time | Date | Name | Meet |
|---|---|---|---|
| 25.32 | Jan. 28, 2023 | Elijah Venos | Denison vs. Ohio Wesleyan vs. Youngstown State |
| 25.73 | Nov. 9, 2020 | Richie Kurlich | Depauw-Wabash Virtual Invite |
| 25.96 | Jan. 30, 2016 | Brendan Howley | Denison vs. Ohio Wesleyan |
| 26.46 | Jan. 31, 2015 | Alex Nennig | Ohio Wesleyan vs. Denison |
| 27.08 | Oct. 12, 2013 | Alex Nennig | Denison vs. Toledo, Wabash |
| 27.85 | Oct. 30, 2010 | Jake Lewing | Denison vs. Ohio Wesleyan |
