| Time | Date | Name | Meet |
|---|---|---|---|
| 49.23 | Feb. 25, 2022 | Regan Smith | Pacific 12 Championships (W) |
| 49.69 | Feb. 24, 2017 | Ally Howe | Pacific 12 Championships (W) |
| 50.71 | Feb. 26, 2016 | Ally Howe | Pacific 12 Championships (W) |
| 50.91 | March 21, 2014 | Felicia Lee | NCAA Division I Womens Championships |
| 51.29 | Feb. 28, 2014 | Felicia Lee | Pacific 12 Championships (W) |
| 51.46 | Nov. 22, 2013 | Felicia Lee | Art Adamson Invitational |
| 51.58 | March 22, 2013 | Felicia Lee | NCAA Division I Womens Championships |
| 51.63 | March 1, 2013 | Felicia Lee | Pacific-12 Womens Championships |
| 51.69 | March 16, 2012 | Betsy Webb | NCAA Division I Womens Championships |
| 52.28 | Feb. 24, 2012 | Betsy Webb | Pacific-10 Conference Womens Championships |
| 52.39 | March 18, 2010 | Betsy Webb | NCAA Division I Womens Championships |
| 52.46 | Feb. 24, 2010 | Betsy Webb | Pacific 10 Women's Championships |
| 52.70 | March 20, 2009 | Laura Wadden | NCAA Division I Womens Championships |
| 52.88 | Feb. 21, 2009 | Betsy Webb | Stanford @California |
| 53.49 | March 9, 2007 | Brooke Bishop | NCAA Division I Womens Championships |
