| Name | Time | Date | Meet |
|---|---|---|---|
| Gia Dalesandro | 50.45 | Feb. 17, 2017 | 2017 Big Ten Conference Championships (W) |
| Gia Dalesandro | 51.75 | Feb. 21, 2014 | 2014 Big Ten Conference Championships (W) |
| Kateryna Zubkova | 52.44 | Feb. 20, 2009 | 2009 Big Ten Championships (Women) - Day Three - Finals |
| Kateryna Zubkova | 53.23 | March 21, 2008 | 2008 NCAA Division I Womens Championships |
| Annica Lofstedt | 55.30 | March 9, 2007 | 2007 NCAA Division I Womens Championships |
