| Name | Time | Date | Meet |
|---|---|---|---|
| Lilly King | 25.98 | March 22, 2019 | NCAA Division I Womens Championships |
| Lilly King | 26.11 | Feb. 22, 2019 | Big Ten Conference Championships (W) |
| Lilly King | 26.40 | Nov. 16, 2018 | Indiana Invitational |
| Lilly King | 27.62 | Oct. 19, 2018 | Texas vs. Florida vs. Indiana |
| Ashlee Specht | 29.07 | Dec. 3, 2010 | USA Swimming Short Course National Championships |
| Sarah Stockwell | 29.45 | March 9, 2007 | 2007 NCAA Division I Womens Championships |
