| Name | Time | Date | Meet |
|---|---|---|---|
| Shelbey Fuller | 31.59 | Feb. 16, 2024 | Great Midwest-Mountain East Conference Championship |
| Shelbey Fuller | 31.77 | Feb. 9, 2023 | Great Midwest-Mountain East Conference Championship |
| Shelbey Fuller | 32.47 | Feb. 18, 2022 | Great Midwest-Mountain East Conference Championship |
| Kenya Paiz | 33.54 | Oct. 21, 2017 | Davis & Elkins vs. Urbana |
