| Name | Time | Date | Meet |
|---|---|---|---|
| Evan Ziolkowski | 45.66 | Feb. 19, 2022 | Southern Athletic Association Championships |
| Evan Ziolkowski | 45.69 | Jan. 22, 2021 | Delta vs. Rhodes |
| Evan Ziolkowski | 46.06 | Feb. 15, 2020 | Southern Athletic Association Championships |
| Will Raines | 46.13 | Feb. 13, 2016 | 2016 Southern Athletic Association Championships |
| Erik Campbell | 46.88 | Feb. 19, 2011 | Southern Collegiate Athletic Conference Championships |
| Erik Campbell | 47.48 | Feb. 20, 2010 | Southern Collegiate Athletic Conference Championships |
| Erik Campbell | 48.82 | Dec. 5, 2009 | DePauw Invitational |
| Jarrett Tate | 48.93 | Jan. 16, 2009 | Washington University Invitational |
| Jarrett Tate | 48.98 | Dec. 5, 2008 | 2008 DePauw Invitational |
