| Name | Time | Date | Meet |
|---|---|---|---|
| Abigail Dolan | 48.27 | March 23, 2019 | NCAA Division I Womens Championships |
| Abigail Dolan | 48.36 | Feb. 17, 2018 | Atlantic Coast Championships (W) |
| Abigail Dolan | 48.70 | Feb. 16, 2017 | 2017 Atlantic Coast Championships (W) |
| Amywren Miller | 49.14 | Feb. 21, 2009 | 2009 Big East Championships - Day Four - Finals |
| Christa Riggins | 50.75 | March 10, 2007 | 2007 NCAA Division I Womens Championships |
| Rebecca Grove | 51.54 | Nov. 20, 2005 | 2005 Minnesota Invitational |
