| Name | Time | Date | Meet |
|---|---|---|---|
| Philip Jensen | 48.34 | Feb. 23, 2024 | Summit League Championships |
| Peter Mullee | 48.63 | March 18, 2010 | 2010 NCAA Division III Championships |
| Peter Mullee | 48.63 | March 18, 2010 | 2010 NCAA Division III Championships |
| Peter Mullee | 48.69 | Feb. 19, 2010 | Minnesota Intercollegiate Athletic Conference Championships |
| Peter Mullee | 48.97 | Feb. 18, 2010 | Minnesota Intercollegiate Athletic Conference Championships |
| Peter Mullee | 49.80 | March 20, 2009 | 2009 NCAA Division III Championships |
