| Name | Time | Date | Meet |
|---|---|---|---|
| Sami Roemer | 57.04 | Dec. 2, 2023 | MO State vs. Mizzou |
| Ulyana ZUBINA | 57.05 | Dec. 3, 2022 | Missouri vs. Missouri State |
| Josie Pearson | 59.73 | Dec. 1, 2017 | Missouri State vs. Evansville |
| Brianna Willoughby | 1:00.35 | Oct. 10, 2009 | Show-Me Showdown |
