| Name | Time | Date | Meet |
|---|---|---|---|
| Elisabeth TIMMER | 56.43 | Jan. 29, 2022 | Coyote Invitational |
| Ashley Theobald | 57.22 | Nov. 23, 2019 | Augustana/Sioux Falls Invitational |
| Kristin Erf | 57.33 | Feb. 17, 2018 | Summit League Championships |
| Ashley Theobald | 57.99 | Feb. 18, 2017 | 2017 Summit League Championships |
| Kristin Erf | 59.68 | Oct. 29, 2016 | South Dakota State vs. South Dakota |
