| Name | Time | Date | Meet |
|---|---|---|---|
| Emma Bachelder | 29.68 | Feb. 24, 2023 | Summit League Championships |
| Alyssa Eckstein | 29.71 | Nov. 22, 2019 | Augustana/Sioux Falls Invitational |
| Samantha Frigard | 30.40 | Feb. 22, 2019 | Summit League Championships |
| Kaitlyn Hemmingson | 31.18 | Jan. 13, 2012 | Iowa Central CC vs South Dakota State |
| Katie Budahl | 31.37 | Nov. 7, 2009 | UW-Green Bay vs. South Dakota State - PM |
| Kim Iverson | 33.55 | Nov. 19, 2005 | 2005 Minnesota Invitational |
