| Name | Time | Date | Meet |
|---|---|---|---|
| Morgan Meyer | 47.55 | Feb. 6, 2019 | Great Lakes Valley Conference Championships |
| Aaron Taske | 47.95 | Dec. 17, 2018 | Delta State Christmas Invitational |
| Eirik Nielsen | 48.11 | Feb. 8, 2018 | Great Lakes Valley Conference Championships |
| Andy Schranck | 48.56 | Dec. 3, 2010 | Missouri Invitational |
| Andy Schranck | 48.92 | March 12, 2009 | 2009 NCAA DII Men's & Women's Championships |
| Travis Stensby | 48.97 | March 15, 2007 | 2007 NCAA Division II Championships |
| Travis Stensby | 49.67 | March 9, 2006 | 2006 NCAA Division II Championships |
