| Name | Time | Date | Meet |
|---|---|---|---|
| Erin Sullivan | 25.37 | March 8, 2017 | 2017 NCAA Division II Championship Festival |
| Erin Sullivan | 25.69 | Feb. 17, 2016 | 2016 New South Conference Championships |
| Erin Sullivan | 26.13 | Feb. 18, 2015 | 2015 New South Conference Championships |
| Annika Bordak | 27.58 | Feb. 6, 2014 | 2014 Northern Sun Intercollegiate Conference Championships |
| Lisa Black | 27.66 | March 11, 2009 | 2009 NCAA DII Men's & Women's Championships |
| Barbie Brooks | 28.19 | March 12, 2008 | 2008 NCAA Division II Championships |
