| Name | Time | Date | Meet |
|---|---|---|---|
| Erin Sullivan | 51.54 | Feb. 10, 2017 | 2017 Northern Sun Intercollegiate Conference Championships |
| Rachel Erickson | 52.59 | Jan. 4, 2014 | Tampa Invitational |
| Jenna Thompson | 52.77 | Feb. 10, 2013 | 2013 Northern Sun Intercollegiate Conference |
| Amy Roness | 53.10 | March 14, 2009 | 2009 NCAA DII Men's & Women's Championships |
| Amy Roness | 53.87 | March 15, 2008 | 2008 NCAA Division II Championships |
