| Name | Time | Date | Meet |
|---|---|---|---|
| Stacy Busack | 47.96 | March 31, 2009 | – |
| Stacy Busack | 48.08 | Feb. 21, 2009 | 2009 Big Ten Championships (Women) - Day Four - Finals |
| Stacy Busack | 49.34 | March 22, 2008 | 2008 NCAA Division I Womens Championships |
| Jenny Shaughnessy | 49.84 | March 10, 2007 | 2007 NCAA Division I Womens Championships |
| Meredith McCarthy | 51.76 | Nov. 20, 2005 | 2005 Minnesota Invitational |
