| Name | Time | Date | Meet |
|---|---|---|---|
| Tevyn Waddell | 24.12 | Feb. 22, 2019 | Big Ten Conference Championships (W) |
| Tevyn Waddell | 24.34 | Nov. 16, 2018 | Hawkeye Invitational |
| Blake Zeiger | 24.91 | Nov. 9, 2012 | Minneapolis Grand Prix |
| Megan Braun | 25.83 | Dec. 3, 2010 | USA Swimming Short Course National Championships |
| Jenny Shaughnessy | 26.27 | Nov. 19, 2005 | 2005 Minnesota Invitational |
