| Name | Time | Date | Meet |
|---|---|---|---|
| Rowan Mayette | 30.53 | Feb. 16, 2024 | Allegheny Mountain / Empire 8 Conference Championships |
| Rowan Mayette | 31.12 | Jan. 14, 2023 | HWS Spring Invitational |
| Rowan Mayette | 31.54 | April 3, 2021 | Oberlin vs. Penn State Behrend vs. Wells |
| Courtney Cloutier | 31.92 | Jan. 23, 2019 | SUNY-Delhi vs. Wells |
| Asa Cade | 40.54 | Nov. 11, 2017 | Cedar Crest vs. Gallaudet, Wells |
