| Name | Time | Date | Meet |
|---|---|---|---|
| Ila Jackson | 27.64 | Feb. 16, 2019 | Landmark Conference Championships |
| Ila Jackson | 28.52 | Oct. 28, 2017 | Elizabethtown vs. Goucher |
| Ila Jackson | 28.90 | Jan. 29, 2016 | Loyola vs. Goucher |
| Victoria Adinolfi | 29.25 | Oct. 22, 2011 | Goucher vs. Susquehanna |
| Victoria Adinolfi | 30.04 | Oct. 24, 2009 | Goucher vs. Susquehanna |
