| Name | Time | Date | Meet |
|---|---|---|---|
| Caroline Lee | 27.56 | Nov. 19, 2022 | Wabash Pebworth Invitational |
| Sarah Albrecht | 27.70 | Feb. 16, 2022 | Southern Athletic Association Championships |
| Connor Foster | 27.82 | Nov. 7, 2020 | Centre vs. VMI, WVU Tech |
| Colleen Maggard | 27.88 | March 21, 2012 | 2012 NCAA Division III Championships |
| Colleen Maggard | 27.96 | March 18, 2009 | 2009 NCAA Division III Championships |
