| Name | Time | Date | Meet |
|---|---|---|---|
| Callie West | 26.01 | Feb. 10, 2023 | Southern Athletic Association Championships |
| Callie West | 26.06 | Nov. 19, 2022 | Wabash Pebworth Invitational |
| Callie West | 26.13 | Nov. 20, 2021 | Gail Moll Pebworth Wabash Inv |
| Annie Holmes | 26.43 | Nov. 23, 2019 | Gail Moll Pebworth Invitational |
| Annie Holmes | 26.53 | Feb. 15, 2019 | Southern Athletic Association Championships |
| Elisabeth Starr | 26.85 | March 9, 2007 | 2007 NCAA Division III Womens Championships |
