| Name | Time | Date | Meet |
|---|---|---|---|
| Callie West | 55.54 | Nov. 19, 2022 | Wabash Pebworth Invitational |
| Callie West | 55.59 | March 17, 2022 | NCAA Division III Championship |
| Callie West | 55.64 | Feb. 18, 2022 | Southern Athletic Association Championships |
| Annie Holmes | 56.09 | Nov. 23, 2019 | Gail Moll Pebworth Invitational |
| Elisabeth Starr | 56.42 | March 19, 2009 | 2009 NCAA Division III Championships |
| Elisabeth Starr | 57.22 | March 9, 2007 | 2007 NCAA Division III Womens Championships |
