| Name | Time | Date | Meet |
|---|---|---|---|
| Madeline Bruce | 54.67 | Feb. 16, 2014 | 2014 Midwest Conference Championships |
| Madeline Bruce | 55.66 | Dec. 6, 2013 | Grinnell College Invitational |
| Victoria Kassabaum | 56.00 | Feb. 20, 2011 | Midwest Conference Championships |
| Victoria Kassabaum | 57.68 | Jan. 15, 2010 | Grinnell Invitational |
| Victoria Kassabaum | 59.69 | Nov. 15, 2008 | 2008 Monmouth Invitational |
