| Name | Time | Date | Meet |
|---|---|---|---|
| Sara Rizzetto | 50.27 | Feb. 19, 2022 | Atlantic 10 Conference Championships |
| Elizabeth Jordan | 50.32 | Feb. 23, 2013 | 2013 Atlantic 10 Conference Championships |
| Elizabeth Jordan | 50.82 | Nov. 18, 2012 | Bucknell Invitational |
| Holly Biro | 51.05 | Feb. 26, 2011 | Atlantic 10 Conference Championships |
| Holly Biro | 51.64 | Feb. 20, 2010 | Atlantic 10 Championships |
| Holly Biro | 51.95 | Feb. 21, 2009 | 2009 Atlantic 10 Championships - Day Four - Finals |
| Shannon Duval | 52.75 | Oct. 24, 2008 | La Salle @Villanova |
