| Name | Time | Date | Meet |
|---|---|---|---|
| Edenna Chen | 27.89 | March 18, 2022 | NCAA Division III Championship |
| Edenna Chen | 28.42 | Feb. 19, 2022 | New England Womens and Mens Athletics Conference Championships |
| Edenna Chen | 28.55 | Dec. 7, 2019 | MIT Winter Invitational |
| Edenna Chen | 29.55 | Nov. 8, 2019 | Yale vs. MIT |
| Amanda Wu | 30.63 | March 20, 2015 | 2015 NCAA Division III Championships |
| Christy Rogers | 30.89 | Jan. 21, 2012 | Tufts vs. MIT |
| Christy Rogers | 30.96 | Nov. 5, 2011 | New England Invitational |
| Kate Thornton | 32.20 | March 10, 2006 | 2006 NCAA Division III Womens Championships |
