| Name | Time | Date | Meet |
|---|---|---|---|
| Olivia McGrath | 25.66 | Feb. 23, 2019 | New England Womens and Mens Athletics Conference Championships |
| Katie Siegel | 25.76 | Jan. 19, 2013 | MIT vs. Tufts |
| Anna Kokensparger | 26.42 | Jan. 14, 2012 | MIT vs. Colby |
| Julianna Edwards | 27.92 | March 10, 2006 | 2006 NCAA Division III Womens Championships |
