| Name | Time | Date | Meet |
|---|---|---|---|
| Emma Sticklen | 49.70 | March 22, 2024 | NCAA Division I Womens Championship |
| Emma Sticklen | 49.79 | Jan. 27, 2023 | Texas vs. NC State (Friday) |
| Emma Sticklen | 50.29 | March 18, 2022 | NCAA Division I Womens Championship |
| Olivia Bray | 50.37 | Dec. 4, 2020 | Texas vs. Texas Christian |
| Kathleen Hersey | 51.40 | Dec. 4, 2008 | 2008 Texas Invitational |
| Katie Robinson | 53.05 | March 9, 2007 | 2007 NCAA Division I Womens Championships |
| Ashley Razo | 55.94 | March 6, 2003 | American Short Course Championships |
